Quality
Understanding HPLC Testing
REPRIME Research·17 Mar 2026·5 min read
What an HPLC chromatogram actually shows — and how to read one with confidence.
High-performance liquid chromatography is the workhorse of peptide purity verification. This short guide explains what the chromatogram represents and what a buyer should look for.
Reading a chromatogram
Each peak represents a compound separated by the column. The main peak is the target peptide; smaller peaks are related substances. The purity percentage is the area-under-curve ratio.
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